Antibodies against Avian influenza virus specific hemagglutinins (H5 un H7 subtypes)
Category: Veterinary investigations
Animal species: Bird
Diesase/Examination: Avian influenza
Type of analysis/testing
Serology
Method/principle
OIE Manual, chapter 2.3.4., Haemagglutination inhibition test (HI)
The method has been accredited
Sample volume, packaging
Not less than 3 ml in vacuum test tube
Turnaround time (business days)
5
Avian influenza and Newcastle disease viruses, hemagglutinins
Category: Veterinary investigations
Animal species: Bird
Diesase/Examination: Avian influenza, Newcastle disease
Type of analysis/testing
Virology
Object
Cloacal and tracheal swab, parenchymal organs, brain, samples of small and large intestines
Method/principle
OIE Manual Terr. ch. 2.3.4., 2.3.14. Virus isolation/Haemagglutination test (VI/HA)
Sample volume, packaging
Send within 48 hours +4 °C, not less than 2 g from each organ in double, sterile waterproof container and/or tracheal, cloacal swab with sterile cotton swab in test tube with transport solution
Turnaround time (business days)
25
Avian influenza and Newcastle disease viruses hemagglutinins serotype
Category: Veterinary investigations
Animal species: Bird
Diesase/Examination: Avian influenza
Object
Cloacal and tracheal swab, parenchymal organs, brain, samples of small and large intestines
Method/principle
OIE Manual Terr. ch. 2.3.4., 2.3.14. Haemagglutination inhibition test (HI)
The method has been accredited
Sample volume, packaging
Send within 48 hours +4 °C, not less than 2 g from each organ in double, sterile waterproof container and/or tracheal, cloacal swab with sterile cotton swab in test tube with transport solution
Turnaround time (business days)
2
Avian influenza virus matrix gene
Category: Veterinary investigations
Animal species: Bird
Diesase/Examination: Avian influenza
Type of analysis/testing
Molecular biology
Object
Cloacal and tracheal swab, stabilized blood, tissue sample
Method/principle
Real time Reverse Transcription Polymerase Chain Reaction
The method has been accredited
Sample volume, packaging
Send within 46 hours (+5±3) °C, not less than 2 g from each organ in double, sterile waterproof container and/or tracheal, cloacal swab with sterile cotton swab in test tube with transport solution
Turnaround time (business days)
3
Avian influenza virus subtype H5, H7, N1 and N8 specific RNA
Category: Veterinary investigations
Animal species: Bird
Diesase/Examination: Avian influenza
Type of analysis/testing
Clinical morphology
Object
Cloacal and tracheal swab, stabilized blood, tissue sample
Method/principle
Real time Reverse Transcription Polymerase Chain Reaction
The method has been accredited
Sample volume, packaging
Send within 46 hours (+5±3) °C, not less than 2 g from each organ in double, sterile waterproof container and/or tracheal, cloacal swab with sterile cotton swab in test tube with transport solution
Turnaround time (business days)
3
Genome sequencing of virus specific genetic material
Category: Veterinary investigations
Animal species: Sheep, Aquaculture, Bee, Deer, Swine, Bovine, Cat, Goat, Fur-bearing animal, Laboratory animal, Bird, Dog, Horse
Diesase/Examination: Rabies, Avian influenza, Newcastle disease, African swine fever, Classical swine fever (Hog cholera), Bluetongue
Type of analysis/testing
Molecular biology
Object
Virus isolates, clinical or tissue samples
The method has been accredited
Sample volume, packaging
Virus containing suspension prepared from clinical or tissue samples; virus isolates after multiplication in chicken embryos or cell cultures under laboratory circumstances (+5±3) °C
Turnaround time (business days)
15
Influenza virus type A virus specific genetic material
Category: Veterinary investigations
Animal species: Bird
Diesase/Examination: Avian influenza
Type of analysis/testing
Molecular biology
Object
Excretion, swab, pathological material
Method/principle
Real time Reverse Transcription Polymerase Chain Reaction (rt RT-PCR)
The method has been accredited
Sample volume, packaging
Not less than 10 g from changed tissues or from each organ, in container or in double package; Blood - not less than 3 ml, in vacuum test tube, (+5±3) °C
Turnaround time (business days)
3