Bacillus anthracis
Category: Veterinary investigations
Animal species: Sheep, Deer, Swine, Bovine, Cat, Goat, Fur-bearing animal, Laboratory animal, Dog, Horse
Diesase/Examination: Anthrax (Siberian plague)
Type of analysis/testing
Microbiology
Object
Blood sample, lymph nodes from ear, tonsil and lower jaw
Sample volume, packaging
Each sample, organ packed separately in waterproof container.
Blood - 1 ml, in a sterile test tube, in metal container with a warning: For investigations of Anthrax, on ice or in cool bag, must be delivered to the place of testing as soon as possible
Turnaround time (business days)
Primary result in 3 days; final result in 10 days
Prion proteins of Transmissible spongiform encephalopathy
Category: Veterinary investigations
Animal species: Sheep, Deer, Bovine, Goat, Fur-bearing animal
Diesase/Examination: Bovine spongiform encephalopathy, Scrapie, Chronic wasting disease (CWD), Transmissible mink encephalopathy
Type of analysis/testing
Histology
Object
Ruminant Medulla oblongata and mink brain
Method/principle
OIE Manual, Chapter 2.4.5., 2.7.12., Classical histology
The method has been accredited
Sample volume, packaging
Brain obex, cerebellar peduncles, tectum of midbrain (quadrigeminal plate). In single-use container, fixed in 10% formalin solution, formalin and sample volume ratio 10:1 at room temperature or fresh (stored at 2-8 °C)
Turnaround time (business days)
4
Type of analysis/testing
Histology
Object
Ruminant and mink brain and lymphoid tissue
Method/principle
OIE Manual, Chapter 2.4.5., 2.7.12., Immunohistochemistry (IHC)
The method has been accredited
Sample volume, packaging
Brain obex, cerebellar peduncles, tectum of midbrain (quadrigeminal plate).
Sheep - in addition - cerebellum. Deer - in addition - lymph nodes.
In single-use container, fixed in 10% formalin solution, formalin and sample volume ratio 10:1 at room temperature or fresh (stored at 2-8 °C)
Turnaround time (business days)
15
Object
Ruminant Medulla oblongata
Method/principle
OIE Manual Terr.ch. 2.4.5., 2.7.12. BIO-RAD TeSeE or IDEXX Herd Check
The method has been accredited
Sample volume, packaging
Brain obex, cerebellar peduncles, tectum of midbrain (quadrigeminal plate) in single-use container
Turnaround time (business days)
1 (Investigations are made in Wednesdays and Fridays)
Specific genetic material of B. anthracis
Category: Veterinary investigations
Animal species: Sheep, Deer, Swine, Bovine, Cat, Goat, Fur-bearing animal, Laboratory animal, Dog, Horse
Diesase/Examination: Anthrax (Siberian plague)
Type of analysis/testing
Molecular biology
Object
Various environmental samples, swabs from animals in affected region
Method/principle
Real time Polymerase Chain Reaction
Sample volume, packaging
Various environmental samples, swabs from animals in affected region (+5±3) °C
Turnaround time (business days)
2