During the fourth stage of the project No. 188.8.131.52/16/A/258 “Development and the application of innovative instrumental analytical methods for the combined determination of a wide range of chemical and biological contaminants in support of the bio-economy in the priority sectors of economy” the advanced instrumental method for the determination of short-chain chlor-alkanes (C10 to C13) was developed using high-performance liquid chromatography combined with a hybrid quadrupole time-of-flight mass spectrometery (HPLC-Q-TOF). Chlor-alkanes is a group of compounds which is classified as a priority hazardous substance in the Water Framework Directive. The emissions and losses of these compounds should be gradually reduced till year 2020, thereby the effective method of detection is essential for achieving this goal. During the study the optimization was performed for both mass spectrometric and chromatographic parameters in order to make the developed method more suitable for the determination of chlor-alkanes. Developed method is competitive with conventional gas chromatographic method and can be used to identify compounds of chlor-alkan groups in both food and environmental samples.
At this stage of the project, studies on the development of the UHPLC-q-Orbitrap method for analysis of multi-residues of veterinary medicines were carried out using various sample preparation methods. This procedure will allow simultaneously to identify and quantify more than 100 residues and metabolites of pharmacologically active substances belonging to different therapeutic classes such as anti-infective agents (antibiotics and chemotherapeutic agents), anti-inflammatory and antiparasitic agents, corticosteroids and agents that act on the nerve and reproductive systems.
In the fourth stage of the project, activities 2 and 4 of the project were started. Within the scope of these activities, according to the methods developed by the project, food and environmental samples testing was performed to determine levels of chemical and biological pollutants and to obtain data for databases.
During the fourth phase of the project, within the scope of development of biologic pollutant methods, the studies have been continued on the detection of salmonella in samples from different stages of the food production chain. In case of outbreak of salmonella, high-resolution methods are required in order to distinguish and classify the isolates as precisely as possible. For this purpose, experts in the molecular biology of the Institute BIOR have in practice introduced the full genome sequenation of salmonella.
In order to ensure the transfer of knowledge gained in the project, three publications are currently being evaluated in the journals. The publication "Brominated flame retardants and toxic elements in meat and liver of deer (Cervus elaphus), wild boar (Sus scrofa) and moose (Alces alces) from Latvian wildlife" is published in the Science of the Total Environment journal, which is included in the Web of Science and Scopus databases.
The total project costs: EUR 449, 617.00 (the planned ERDF aid (85%): EUR 382, 174, 44 and state budget aid (7, 5%): EUR33721, 28). Implementation of the project is scheduled at the duration of thirty months (period: 01.03.2017. - 30.08. 2019).
Information prepared on 28.03.2018.